Intracytoplasmic Sperm Injection (ICSI) is an in vitro fertilization procedure in which a single sperm cell is injected directly into the cytoplasm of an egg. 


Intracytoplasmic Sperm Injection (ICSI) is usually referred as micro manipulation is a useful method for treating severe male factor infertility patients. 
In this procedure the single sperm injected into an egg,then the fertilized embryo is transferred to the women’s uterus. This procedure mainly effective for men who have very poor sperm count.

  • Involves injecting a single sperm into an egg
  • Fertilized egg is transferred to women’s uterus
  • Solution to irreversible infertility in men


IVF - Intracytoplasmic Sperm Injection (ICSI)

Sperm defects are the most common cause of failure of fertilisation in clinical IVF. With the use of intracytoplasmic sperm injection (ICSI), a specialised technique for injecting a single sperm into an egg, many of these sperm can achieve normal fertilization. Sperm analysis before you begin assisted reproductive technology (ART) treatment is important to help your fertility specialist recommend the most appropriate ART technique (IUI, IVF or ICSI).

In patients suffering with obstructive Azoospermia sperms can be obtained directly from the testis by doing an epididymal sperm aspiration or biopsy and the sperms can be used for fertilizing the oocytes.

In ICSI, the entire process is the same as IVF till egg collection. Once the eggs are retrieved, the sperm is directly injected into the egg.

It is a tool of assisted reproductive technology against infertility. Eggs are removed from a woman's ovaries, and placed in one of the Fallopian tubes, along with the man's sperm. With the advances in IVF the GIFT procedure is used less as pregnancy rates in IVF tend to be equal or better and do not require laparoscopy when the egg is put back.

Cryo Preservation of Embryos :

Sometimes when you undergo IVF/ ICSI treatment more than the required embryos will be produced. The excess embryos can be preserved in liquid nitrogen for long periods. Such embryos can be used for future transfer either to have another child or if the cycle fails for any reasons. These Cryo preserved embryos can be conveniently utilized without any hyper stimulation again. Therefore embryo Cryo preservation reduces the cost of treatment and it can be used as and when required.

But one must know that all the Cryo preserved embryos cannot be preserved with life. Pregnancy can be achieved only with embryos with life. However,it is a convenient procedure to the patients who get more number of oocytes (more than required for a single transfer).

Sperm cryopreservation or sperm freezing is a method for men to preserve their sperm and store it in a bank for future use. Storing your sperm allows you to use it for treatment in the future. You may want to consider sperm freezing if:

  • You are about to undergo a vasectomy
  • You have a low sperm count or your sperm quality is deteriorating
  • You have difficulty producing a sample on the day of treatment
  • Your sperm is going to be used for donation
  • You have a medical condition that may result in infertility later in life
  • You are facing medical treatment that may affect your fertility

In patients suffering with obstructive azoospermia sperms can be obtained directly from the testes by doing an epididymal sperm aspiration or biopsy and the sperms can be used for fertilizing the oocytes. The biopsy material can also be preserved in liquid nitrogen for subsequent use.

Firm Hospitals have facilities for conducting the testicular biopsy and semen banking for such patients. We generally take 3-4 biopsy samples and preserve them in liquid nitrogen for long term storage. This means that patients can undergo ICSI, the second time if they want to have a second child.

  • Evaluating family history and medical records
  • Ordering genetic tests
  • Evaluating the results of this investigation
  • Helping parents understand and reach decisions about what to do next

Genetic tests are done by analyzing small samples of blood or body tissues. They determine whether you, your partner, or your baby carry genes for certain inherited disorders. It's especially important to consider genetic counseling if any of the following risk factors apply to you:

  • A standard prenatal screening test (such as the alpha fetoprotein test) yields an abnormal result
  • An amniocentesis yields an unexpected result (such as a chromosomal defect in the unborn baby)
  • Either parent or a close relative has an inherited disease or birth defect
  • Either parent already has children with birth defects, intellectual disabilities, or genetic disorders
  • The mother-to-be has had two or more miscarriages or babies that died in infancy
  • The mother-to-be will be 35 or older when the baby is born. Chances of having a child with Down syndrome
  • Increase with the mother's age: a woman has about a 1 in 350 chance of conceiving a child with Down syndrome at age 35, a 1 in 110 chance at age 40, and a 1 in 30 chance at age 45.
  • You are concerned about genetic defects that occur frequently in certain ethnic or racial groups.
  • Either parent is concerned about the effects of exposures they have had to radiation, medications, illegal drugs, infections, or chemicals.

Our Genetic counselor have completed a master's program in medical genetics and counseling skills.Genetic counselor can help identify and interpret the risks of an inherited disorder, explain inheritance patterns, suggest testing, and lay out possible scenario. He will explain the meaning of the medical science involved, provide support, and address any emotional issues raised by the results of the genetic testing.

Our andrology laboratory provides a full range of analytical services, including routine semen analysis and testing for anti-sperm antibodies.

The andrology laboratory also provides sperm freezing and storage for use in future ART treatment. The laboratory also stores sperm for use in the donor program, or before chemotherapy or radiotherapy where a man’s treatment may affect his sperm function or production in the future.

  • Routine sperm evaluation.
  • Sperm processing.
  • DNA Fragmentation.
  • Sperm morphology
  • Sperm freezing (Sperm Cryo Preservation).
  • Hormonal assay.

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